原啓文 博士(工学) (長岡技術科学大学)

 contact-academic-hirofumi-hara

准教授
環境グリーン工学科, EGT.
Email: hhara@utm.my
Tel: 03 2203 1264

研究分野・研究キーワード

  • 環境微生物学
  • 応用微生物学
  • バイオインフォマティクス

外部資金等

科学研究費補助金

  • リグニン分解菌のプロトカテク酸メタ開裂系酵素遺伝子群の構造・機能・転写制御、研究代表者、特別研究員奨励費(DC1)、平成12年度〜平成14年度
  • ロドコッカス属細菌における難分解性芳香族化合物分解制御系の網羅的解析、研究代表者、若手(スタートアップ)平成20年度〜平成21年度
  • ロドコッカス属細菌における二次代謝産物生合成遺伝子群の誘導条件の探索とその応用、研究代表者、挑戦的萌芽研究、平成22年度〜平成24年度、
  • マレーシア汚濁淡水源におけるエコゲノミクスを用いた浄水処理障害微生物の実態調査、研究分担者、基盤研究(B海外)、平成26年度〜平成28年度、
  • 藍藻産生毒素分解菌の分子育種株を用いた新規水環境修復技術の開発、研究分担者、基盤研究(B)、平成 27 年度〜平成 29 年度、

受託研究費

  • 細菌のリグニン分解酵素遺伝子による植物細胞壁改変技術の開発研究代表者 共同提案、新エネルギー・産業技術総合開発機構(NEDO) 新エネルギー技術開発/バイオマスエネルギー等高効率転換技術開発(先導技術開発)、平成21年度〜平成24年度、

マレーシア国内での研究費

  • Exploring
    of lignin degrading Actinomycetes in Malaysia, Principal investigator,
    UTM Tier 2 E, UTM research grant, April 2014 to March 2015,
  • Development
    scenarios of low carbon society for asian region, UTM Low carbon Asia
    research centre, co-investigator, OTR Research grant from Ministry of
    Education, Malaysia, January 2015 to March 2016,
  • Green
    sonochemical synthesis of silver nanoparticles using chitosan as natural
    polymer media, PAS, UTM research grant, co-investigator, July 2015 to
    July 2016, 20,000 RM (600,000
    )
  • Treatment
    of Klang River Water using Agricultural Waste as Adsorbent, PAS, UTM
    research grant, co-investigator, January 2015 to December 2015,
  • An
    exploratory study of river water treatment using different adsorbents
    for the removal of recalcitrant organic compounds, nutrients and oil and
    grease. Tier 1, UTM research grant, co-investigator, January 2015 to
    December 2015,
  • Pagoh
    project: Development of novel bio-composting technology and production
    of animal feedstock by scw pretreated domestic waste, SJT Research grant
    from Ministry of Education, Malaysia, Principal investigator, Aug 2015
    to July 2017,

職歴

  • 20004月〜20033月 日本学術振興会特別研究員(DC1)
  • 20034月〜20063月 カナダ、ブリティッシュ・コロンビア大学 微生物・免疫学部 博士研究員
  • 20064月〜20083月 東京大学大学院 農学生命科学研究科 産学官連携研究員
  • 20084月〜20123月 岡山理科大学工学部生体医工学科 講師
  • 20124月〜20133月 岡山理科大学工学部生体医工学科 准教授

学会活動等

  • H21H22 日本農芸化学会中四国支部評議委員 2009年~2011 年度
  • H23〜現在 日本生物工学会西日本支部役員(編集幹事) 2011年~2012 年度
  • 日本農芸化学会会員
  • 日本生物工学会会員

主な論文等

論文

  • Takahashi, K., Y. Hirose, N. Kamimura, S.
    Hishiyama, H. Hara, T. Araki, D. Kasai, S. Kajita, Y.
    Katayama, M. Fukuda and E. Masai, Membrane-associated
    Glucose-Methanol-Choline Oxidoreductase Family Enzymes, PhcC and PhcD,
    are Essential for Enantioselective Catabolism of Dehydrodiconiferyl
    Alcohol. Appl. and Environ. Microbiol., Accepted for publication,
    (2015)
  • Yamanashi, T,, SY Kim, H. Hara
    and N. Funa, In vitro reconstitution of the catabolic reactions
    catalyzed by PcaHG, PcaB, and PcaL: the protocatechuate branch of the
    β-ketoadipate pathway in Rhodococcus jostii RHA1, Bioscience,
    Biotechnology, Biochemistry
    , 79 (5), 830-835, (2015),
  • Tsuji. Y., R. Vanholme, Y. Tobimatsu, Y.
    Ishikawa, C. E Foster, N. Kamimura, S. Hishiyama, S. Hashimoto, A.
    Shino, H. Hara, K. Sato
    Izawa,
    P. Oyarce, G. Goeminne, K. Morreel, J. Kikuchi, T. Takano, M. Fukuda, Y.
    Katayama, W. Boerjan, J. Ralph, E. Masai, S. Kajita, Introduction of
    chemically labile substructures into Arabidopsis lignin through the use
    of LigD, the Cα
    dehydrogenase from Sphingobium
    sp. strain SYK
    6, Plant Biotechnology
    Journal
    , 13(6):821-32, (2015)

·
Takahashi,
Y., M. Shintani, N. Takase, Y. Kazo, F. Kawamura, H. Hara, H.
Nishida, K. Okada, H.Yamane, H. Nojiri, Modulation of primary cell function
of host Pseudomonas bacteria by the conjugative plasmid pCAR1,
Environ. Microbiol
, 17 (1), 134-155, 2015,

·
Takahashi,
K., N. Kamimura, S. Hishiyama, H. Hara, D. Kasai, Y. Katayama,
M. Fukuda, S. Kajita, and E. Masai. Characterization of the catabolic pathway
for a phenylcoumaran-type lignin-derived biaryl in Sphingobium sp.
strain SYK-6. Biodegradation, 25(5):735-745, (2014). 

·
Tamura, M.,
Y. Tsuji, T. Kusunose, A. Okazawa, N.i Kamimura, T. Mori, R. Nakabayashi, S.
Hishiyama, Y. Fukuhara, H. Hara, K. Sato, T. Muranaka, K.
Saito, Y. Katayama, M. Fukuda, E. Masai, and S. Kajita, Successful expression
of a novel bacterial gene for pinoresinol reductase and its effect on lignan
biosynthesis in transgenic Arabidopsis thaliana, Applied Microbiology
and Biotechnology, 98(19):8165-8177, (2014).

  • Torii,
    H., A. Machida, H. Hara, T. Hatta and N. Takizawa: The
    regulatory mechanism of 2,4,6-trichlorophenol catabolic operon
    expression by HadR in Ralstonia pickettii DTP0602. Microbiology,
    159(4), 665-677. (2013)
  • Fukuhara,
    Y., N. Kamimura, M. Nakajima, S. Hishiyama, H. Hara, D.
    Kasai, Y. Tsuji, S. Narita-Yamada, S. Nakamura, Y. Katano, N. Fujita, Y.
    Katayama, M. Fukuda, S. Kajita, and E. Masai: Discovery of pinoresinol
    reductase genes in Sphingomonads. Enzyme and Microbial Technology.
    52(1): 38-43. (2013)
  • Higo,
    A., H. Hara, S. Horinouchi, and Y. Ohnishi: Genome-wide
    distribution of AdpA, a global regulator for secondary metabolism and
    morphological differentiation in Streptomyces, revealed the
    extent and complexity of the AdpA regulatory network. DNA research,
    19(3), 259–273, (2012)
  • Masai,
    E., N. Kamimura, D. Kasai, A. Oguchi, A. Ankai, S. Fukui, M. Takahashi,
    I. Yashiro, H. Sasaki, T. Harada, S. Nakamura, Y. Katano, S.
    Narita-Yamada, H. Nakazawa, H. Hara, Y. Katayama, M.
    Fukuda, S. Yamazaki, and N. Fujita: Complete genome sequence of
    Sphingobium sp. strain SYK-6, a degrader of lignin-derived biaryls and
    monoaryls, Journal of Bacteriology, 194(2) 534-535 (2012)
  • Kamimura,
    N., K. Takamura, H. Hara, D. Kasai, R. Natsume, T. Senda,
    Y. Katayama, M. Fukuda, and E. Masai: Regulatory system of the
    protocatechuate 4,5-cleavage pathway genes essential for lignin
    downstream catabolism, Journal of Bacteriology, 192(13) 3394-3405 (2010)
  • Shintani,
    M., Y. Takahashi, H. Tokumaru, K. Kadota, H. Hara, M.
    Miyakoshi, K. Naito, H. Yamane, H. Nishida, and H. Nojiri: Response of
    the Pseudomonas host chromosomal transcriptome to carriage of the
    IncP-7 plasmid pCAR1, Environmental Microbiology, 12(6) 1413-1426 (2010)
  • Hara,
    H
    ., G. R.
    Stewart, and W. W. Mohn: Involvement of a novel ABC transporter and
    monoalkyl phthalate ester hydrolase in phthalate ester catabolism by Rhodococcus
    jostii
    RHA1. Applied and Environmental Microbiology, 76(5) 1516-1523
    (2010)
  • Akanuma,
    G., H. Hara, Y. Ohnishi, and S. Horinouchi: Dynamic
    changes in the extracellular proteome caused by absence of a pleiotropic
    regulator AdpA in Streptomyces griseus. Molecular Microbiology.,
    73(5) 898-912 (2009)
  • Tezuka,
    T., H. Hara, Y. Ohnishi, and S. Horinouchi: Identification
    and gene disruption of small noncoding RNAs in Streptomyces griseus.
    Journal of Bacteriology, 191(15) 4896-4904 (2009)
  • Hara,
    H.
    ,
    Y. Ohnishi, and S. Horinouchi: DNA microarray analysis of global gene
    regulation by A-factor in Streptomyces griseus. Microbiology.
    155(7) 2197-2210 (2009)
    ,
  • Ohnishi,
    Y., J. Ishikawa, H. Hara, H. Suzuki, M. Ikenoya, H. Ikeda,
    A. Yamashita, M. Hattori, and S. Horinouchi: Genome sequence of the
    streptomycin-producing microorganism Streptomyces griseus
    IFO13350. Journal of Bacteriology, 190(11) 4050-4060 (2008)
  • Masai,
    E., Y. Yamamoto, T. Inoue, K. Takamura, H. Hara, D. Kasai,
    Y. Katayama and M Fukuda: Characterization of ligV essential for
    catabolism of vanillin by Sphingomonas paucimobilis SYK-6.
    Bioscience Biotechnology and Biochemistry. 71(10) 2487-2492 (2007)
  • van
    der

    Geize, R., K. Yam, T. Heuser, M. H. Wilbrink, H. Hara, W.
    C. Anderton, E. Sim, L. Dijkhuizen, J. E. Davies, W. W. Mohn, and L. D.
    Eltis: A gene cluster encoding cholesterol catabolism in a soil
    actinomycete provides insight into Mycobacterium tuberculosis
    survival in macrophages. Proceedings of the National Academy of Science.
    USA. 104(6) 1947-1952 (2007)
  • Hara,
    H.
    ,
    L. D. Eltis, J. E. Davies, and W. W. Mohn: Transcriptomic analysis
    reveals a bifurcated terephthalate degradation pathway in Rhodococcus
    sp. RHA1. Journal of Bacteriology, 189(5) 1641-1647 (2007)
  • McLeod,
    M. P., R. L. Warren, N. Araki, W. W. L. Hsiao, M. Myhre, C. Fernandes,
    D. Miyazawa, W. Wong, A. L. Lillquist, D. Wang, M. Dosanjh, H.
    Hara
    , A. Petrescu, R. D. Morin, G. Yang, J. M. Stott, J. E.
    Schein, H. Shin, D. Smailus, A. S. Siddiqui, M. A. Marra, S. J. M.
    Jones, R. Holt, F. S. L. Brinkman, K. Miyauchi, M. Fukuda, J. E. Davies,
    W. W. Mohn, and L. D. Eltis: The complete genome of Rhodococcus
    sp. RHA1: insights into a catabolic powerhouse. Proceedings of the
    National Academy of Science. USA. 103(42) 15582-15587 (2006)
  • Goncalves,
    E. R., H. Hara, D. Miyazawa, J. E. Davies, L. D. Eltis, W.
    W. Mohn: Transcriptomic assessment of isozymes in the biphenyl pathway
    of Rhodococcus sp. strain RHA1. Applied and Environmental Microbiology,
    72(9) 6183-6193. (2006)
  • Sasoh,
    M., E. Masai, S. Ishibashi, H. Hara, N. Kamimura, K.
    Miyauchi, M. Fukuda. 2006. Characterization of the terephthalate
    degradation genes of Comamonas sp. strain E6. Applied and
    Environmental Microbiology, 72(3) 1825-1832 (2006)
  • Hara,
    H.
    ,
    E. Masai, K. Miyauchi, Y. Katayama, and M. Fukuda: Characterization of
    4-carboxy-4-hydroxy-2-oxoadipate aldolase gene and operon structure of
    the protocatechuate 4,5-cleavage pathway genes in Sphingomonas
    paucimobilis
    SYK-6. Journal of Bacteriology, 185(1) 41-50 (2003)
  • Hara,
    H.
    ,
    E. Masai, Y. Katayama, and M. Fukuda: The 4-oxalomesaconate hydratase
    gene, involved in the protocatechuate 4,5-cleavage pathway, is essential
    to vanillate and syringate degradation in Sphingomonas paucimobilis SYK-6.
    Journal of Bacteriology, 182(24) 6950-6957. (2000)
  • Masai,
    E., K, Momose, H. Hara, S. Nishikawa, Y. Katayama, and M. Fukuda.
    2000. Genetic and biochemical characterization of
    4-carboxy-2-hydroxymuconate-6- semialdehyde dehydrogenase and its role
    in the protocatechuate 4,5-cleavage pathway in Sphingomonas
    paucimobilis
    SYK-6. Journal of Bacteriology, 182(23) 6651-6658
    (2000),

·
Masai,
E., S. Shinohara, H. Hara, S. Nishikawa, Y. Katayama, and M. Fukuda.
1999. Genetic and biochemical characterization of a 2-pyrone-4,6-dicarboxylic
acid hydrolase involved in the protocatechuate 4, 5-cleavage pathway of Sphingomonas
paucimobilis
SYK-6. Journal of Bacteriology, 181(1) 55-62 (1999),

著書

·
岩本 浩二・ 啓文、石崎 浩之、淡水性有用藻類・微生物の海水利用による生産技術、ケミカルエンジニヤリング、20151月号

·
Hirofumi
Hara
,
Engineering new biocatalyst, Forging ahead – the Asia-Pacific research
revolution, International Innovation, Issue 143, p54-56, 2014

·
啓文、大西康夫、福田雅夫: ゲノム情報を基盤にした放線菌群の転写制御機構研究、化学と生物、vol. 49, No. 4, 234-242, 20114月号

·
大西康夫、 啓文、堀之内末治: 微生物ホルモンのケミカルジェネティクス 細胞工学、vol. 28No.
4
360−36520094月号

特許

  • 梶田真也、政井英司、菱山正二郎、原啓文 「新規ピノレジノールレダクターゼ遺伝子および当該遺伝子を導入した形質転換植物」特願2010-287625 平成221224